PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .
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Finally the seeds were rinsed 3 times with sterile distilled water. Lower concentration of TDZ promoted direct shoot regeneraration and higher concentrations promoted callus induction in Hagenia abyssinica Bruce J. Various explants have been used for direct shoot formation.
Callus with good and compact structure was obtained when explant was cultured on MS medium supplemented with the combination of BAP and 2, 4-D. Takayama and Misawa reported that medium containing 0. Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort.
Abstract Regeneration potentials in Gerbera jamesonii Bolus ex. Gerbera jamesonii Bolus ex. High concentrations of cytokinin in culture medium were found to be unsuitable for shoot formation from leaf or petiole explants in some ornamentals.
Tissues of explants generally show distinct planes of cell division, various specializations of cells and organization into specialized structures such as the vascular system.
Many temperate and tropical plants have been successfully propagated via tissue culture. Adventitious organogenesis or shoot formation is a preferred system as it enables to retain the clonal fidelity since gervera ornamental species are cultivars that are propagated for one or more unique characteristics Kantia and Kothari, Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture.
The use of an in vitro adventitious bud technique for mutation breeding of Begoniaxhiemalis. Regenerated plants were rooted on Murashige and Skoog basal medium. Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from Gerbera petioles.
Growth Optimization and Organogenesis of Gerbera jamesonii Bolus ex. Tissue culture technique has long been used since the cell theory was established. In vitro regeneration of Hagenia abyssinica Bruce J. This technology is being utilized commercially in the ornamentals industry and in other plant production organizations worldwide Chu, ; Huetteman and Preece, ; Mantell et al. It is reported that a good combination of cytokinin and auxin in the culture medium enhanced good shoot formation and plantlet regeneration from explants, for example, adventitious Gerbera shoots were regenerated from flower buds of greenhouse grown plants Pierik et al.
Bigot reported that the addition of g L -1 activated charcoal in the culture medium showed vigorous rooting from excised shoots of Begoniax hiemalis. This research was funded by Vote F Grant No.
Genotype is one of the most influential factors on the response of gerbera in vitro. Gefbera effects of various concentrations of plant growth regulators on the multiplication of shoots were examined. The combination of low concentration of cytokinin and auxin initiated propagation of Begonia species Reuter and Bhandari, Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro.
A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plantlets survival rate achieved was Explants were obtained from 8-week-old aseptic seedlings.
Micropropagation of Gerbera (Gerbera jamesonii Bolus).
herbera A potent cytokinin for woody plant tissue culture. In Begoniathe addition of kinetin and zeatin in the culture medium Murashige and Skoog, induced multiple shoots Jain, Combination of auxin and cytokinin induces the formation of adventitious shoots and roots.
The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support. One of the most important techniques in plant improvements is via micro propagation. Today, tissue culture technique is being used widely realizing its potentials in mass propagation and preservation of elite plants. Kluwer Academic Publishers, Dordrecht, pp: Shoots and roots of chrysanthemum were developed on a single medium containing 1.
Highest numbers of shoots from petiole explants 9. Petioles and leaves obtained from aseptic young plantlets formed from the seedlings were used as source of explants. Petiole explants have been identified as the most regenerative explants gerbea multiple shoot formation.
Organogenesis and histogenesis of adventitious organ induced on leaf blade grebera of Begonia elatior hybrids Begonia x hiemalis in tissue culture. Ornamental plants and woody plant species are also propagated commercially by axillary bud proliferation Mantell et al.
Optimilization of Gerbera plantlet production from excised capitulum explants. In conclusion, the research done has proven that micro propagation of Gerbera jamesonii Micro;ropagation ex. Petiole segment was identified as the best explant for regeneration of this species.
Micropropagation of ornamental plants-cut flowers. In vitro establishment and plantlet regeneration of Gerbera jamesonii Bolus ex. Gerbera plantlets formed were transferred to soil with Many scientists have tried to prove the totipotency concept, which is the ability of a single cell to form complete individual.
In Gerbera jamesoniiBAP was also required for the formation of callus. They are planted outdoors in full sun and useful as cut flowers, pot plant and also bedding plant. Relatively, in this experiment, callus formation was optimum when leaf explant was cultured on MS medium supplemented with 1.
The propagation rates via organogenesis can be much higher than axillary shoot proliferation Chun,